DNA damage response during mouse oocyte maturation

Mitochondrial Dynamics During Mouse Oocyte Maturation

Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation.

Pyruvate dehydrogenase kinases (PDKs) modulate energy homeostasis in multiple tissues and cell types, under various nutrient conditions, through phosphorylation of the α subunit (PDHE1α, also known as PDHA1) of the pyruvate dehydrogenase (PDH) complex. However, the roles of PDKs in meiotic maturation are currently unknown. Here, by undertaking knockdown and overexpression analysis of PDK paralo...

Polarity and asymmetry during mouse oogenesis and oocyte maturation.

Cell polarity and asymmetry play a fundamental role in embryo development. The unequal segregation of determinants, cues, and activities is the major event in the differentiation of cell fate and function in all multicellular organisms. In oocytes, polarity and asymmetry in the distribution of different molecules are prerequisites for the progression and proper outcome of embryonic development....

Symmetry breaking and polarity establishment during mouse oocyte maturation.

Mammalian oocyte meiosis encompasses two rounds of asymmetric divisions to generate a totipotent haploid egg and, as by-products, two small polar bodies. Two intracellular events, asymmetric spindle positioning and cortical polarization, are critical to such asymmetric divisions. Actin but not microtubule cytoskeleton has been known to be directly involved in both events. Recent work has reveal...

Cytogenetic effects of caffeine during in vivo mouse oocyte maturation.

Numerous investigators have studied the reproductive and genetic toxicity of caffeine. Caffeine has also been reported to retard meiotic progression and induce aneuploidy in hamster oocytes in vitro. However, the ability of caffeine to induce aneuploidy in mammalian oocytes in vivo has not been reported. The objective of this study was to test the hypothesis that chemical-induced perturbations ...